restriction enzymes
英 [rɪˈstrɪkʃn ˈɛnzaɪmz]
美 [rɪˈstrɪkʃn ˈɛnˌzaɪmz]
网络 限制性内切核酸酶; 限制性内切酶; 限制酶; 限制性核酸内切酶; 限制性酶
双语例句
- They also discovered "scissors," called restriction enzymes for cutting the DNA into predictable, reproducible patterns.
科学家们还发现了被称为限制酶的“剪刀”，可以把DNA分裂成可以预测和可以复制的形式。 - Restriction enzymes occur in bacteria as part of a natural defense mechanism to guard against invading viruses.
作为抵抗病毒的自然防御机制的一部分，限制酶存在于细菌中。 - New phages can develop by acquiring restriction enzymes from plasmids.
通过从质粒获得的限制性酶，新的噬菌体能够生长。 - Restriction enzymes and reverse transcriptase are keys to gene engineering.
限制性酶和逆转录酶成为基因工程的重要手段。 - The method uses fluorescence microscopy to image individual DNA molecules that have been divided into orderly fragments by so-called restriction enzymes.
该方法应用荧光显微术使单个DNA分子成像，这些单个的DNA分子已经被分成有序的片断，因而被称为限制性内切酶的识别序列。 - For genetic manipulation, the Ti plasmid is modified by the removal of the genes within the T-DNA region using restriction enzymes that cut DNA at specific nucleotide sequences.
为了利于基因操作，用限制酶在特定的核苷酸序列处除去T-DNA区内部的基因以进行质粒修饰。 - If different molecules are cut with the same restriction enzymes, the ends produced at each one have the capacity to bind to each other, joining together the two different molecules.
如果不同的分子用相同的限制酶切割，从每个分子产生的末端都能互相结合，从而将两种不同的分子连接在一起。 - In this article, different DNA source, primers and restriction enzymes for CYP2A6 genotypes were reviewed.
本文列举了检测CYP2A6基因型的不同的DNA来源、引物和限制性内切酶。 - When the chromosome was isolated direct from the bacterial cells it was likely already methylated and therefore transplantable due to it being protected from the cells restriction enzymes.
当提取出来的染色体很可能已经被甲基化，因此对其移植应该已经有针对细胞内限制性内切酶的保护。 - Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.
首先用限制性内切酶将大的DNA分子切断，产生出特殊的片段。 - Zinc finger nucleases ( ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme.
锌指核酸酶是一种人工制做限制性内切酶，通过将锌指DNA结合区与限制性内切酶的DNA切割区融合获得。 - 60 Recombinants were digested with 3 restriction enzymes.
用三种识别六碱基的限制性内切酶两两组合消化重组质粒。 - Restriction enzymes was used to identify the specificity of RT PCR products.
用酶切鉴定其产物特异性。 - Study on cloning of Mycoplasma pneumoniae DNA digested with double restriction enzymes
双酶切法克隆肺炎支原体DNA的研究 - Analysis of higher basidiomycetous mitochondrial DNA with four-gc-cutter restriction enzymes
用识别四个GC对的限制酶研究高等担子菌线粒体DNA - The recombinant plasmids were identified by using restriction enzymes and then sequenced.
重组质粒通过限制性内切酶鉴定分析并测序。 - DNA extracted by the method could be digested by restriction enzymes and used as template for PCR.
提取的DNA可用于限制性内切酶酶切和PCR扩增。 - Artificial nucleases are compounds that cleave nucleic acids and function as natural restriction enzymes with high activity and selectivity.
人工核酸酶是一类具有限制性内切酶的功能、能高效高选择性地催化水解DNA或RNA的断裂工具。 - They consist of nucleic acid recognition and catalytic scission systems, and can mimic the natural restriction enzymes by combining these two functions.
它们一般由核酸结构识别系统及催化断裂系统组成，将两种功能有效地结合起来，可模拟核酸的酶切反应。 - This model that based on the theory of splicing systems extends contextual insertions/ deletions model from linear molecular to circular plasmids, and simulates the insertion/ cutting operation with single restriction enzymes.
该模型基于上下文的插入/删除模型，将线性分子拓展到环形质粒分上进行讨论.以剪接系统理论为基础，对在单一限制性内切酶作用下的插入/切割操作进行模拟。 - Different restriction enzymes digestion and Southern hybridization indicated that this sequence was organized in tandem array and dispersed in rice genome.
不同限制性内切酶消化和Southern杂交分析显示，这段重复DNA顺序以串联加散布的形式存在于水稻基因组中； - The fragment was cloned into pET-14b vector and identified by digestion with restriction enzymes and sequence analysis.
利用酶切、序列分析鉴定克隆正确性。 - At last, the ligase dependency of type II restriction enzymes when performing DNA automaton computing was investigated based on the experiment result.
最后，在实验结果的基础上探讨了DNA自动机硬件对连接酶的依赖性。 - The results show that these complexes have the same catalysis activity as restriction enzymes.
结果还发现这些金属配合物具有与限制性内切酶同等优良的催化性能。 - This mainly includes the digestion efficiencies of the vector by restriction enzymes, the cloning efficiency of the PCR products and the transformation efficiency.
主要是各种限制性内切酶的酶切效率、PCR产物的克隆效率、转化效率。 - Digestion of recombinant plasmid with restriction enzymes showed that we succeeded in building a recombinant expression plasmid pET-HNa containing the antigenic determinant fragment of the HN gene.
通过重组质粒的酶切鉴定，表明我们成功构建了包含HN基因主要抗原位点的重组表达质粒pET-HNa。 - After systematically study on the main factors involved, an AFLP silver-staining technique system fitting for persimmon genomic DNA analysis was established. Restriction digestion of genomic DNA was performed using two restriction enzymes.
2通过对各主要影响因素的研究，建立了适于柿树AFLP分析的银染技术体系。